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Servicebio Inc diamidino 2 phenylindole dapi staining
Diamidino 2 Phenylindole Dapi Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+dapi/2+4+6+diamidino+phenylindole/pmc13049910-67-22-29
Average 86 stars, based on 1 article reviews
diamidino 2 phenylindole dapi staining - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Staining:

Article Title: Lactoferrin Deficiency During Lactation Causes Adult Obesity-Related Metabolic Disease Through Persistent Adipose Dysfunction Driven by Impaired Adipocyte Development.
Article Snippet: .. Cell nuclei were stained with DAPI (Servicebio, Wuhan, China, Cat. No: G1012), and cell membranes were labeled with Wheat Germ Agglutinin (WGA) (Sigma, USA, Cat. No: L4895) Crown-like structures (CLS) in adipose tissue were analyzed using ImageJ. ..

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Article Title: Temperature-dependent alterations in skeletal muscle during the postmortem interval: Structural, metabolic, and lncRNA expression changes.
Article Snippet: Background: Estimation of the postmortem interval (PMI) remains a challenge in forensic practice.. Although environmental temperature is known to influence postmortem biological processes, its effects on structural, metabolic, and transcriptional changes in skeletal muscle remain poorly characterized.. Methods: Male C57 mice were euthanized and carcasses were maintained at 30 C, 20 C, or 4 C. Skeletal muscle samples were collected at 0, 4, 8, 12, 24, and 36 h postmortem.

Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
Article Snippet: Polysorbate 80 (Tween 80) was obtained from Adamas-Beta Co., Ltd. (Shanghai, China). .. Citrate antigen retrieval buffer (pH 6.0), EDTA antigen retrieval buffers (pH 8.0 and pH 9.0), phosphate-buffered saline (PBS), tissue autofluorescence quencher, and 4′,6-diamidino-2-phenylindole (DAPI) staining reagent were provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. Propidium iodide (PI) and Calcein-AM were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China).

Article Title: Vincamine attenuates alcoholic liver injury through modulation of a CDK1-glycolysis-NLRP3 immunometabolic axis.
Article Snippet: Background: Alcoholic liver disease (ALD) is the major immunometabolic disorder characterized by dysregulated lipid metabolism and persistent activation of hepatic innate immune responses.. Vincamine (Vin), isolated from Vinca minor, has been reported to exhibit diverse pharmacological activities.. Nevertheless, the immunomodulatory effects and underlying mechanisms of Vin on ALD remain poorly understood.

Article Title: Sensory nerve-derived semaphorin 3A promotes bone healing through macrophage immunomodulation.
Article Snippet: Sensory innervation has emerged as a crucial regulator of skeletal homeostasis and regeneration.. Growing evidence indicates that disruption of sensory nerves is associated with excessive inflammation and impaired tissue healing.. However, the underlying mechanisms by which neuronal signaling regulates the immune microenvironment to orchestrate bone repair remain unclear.

Article Title: Evolutionary conservation and divergence of phagocytic and coagulation programs across bilaterian circulating immune cells.
Article Snippet: .. After being washed by the following SSC: 2 × SSC, 37°C 3 for 10 min, 1 × SSC, 37°C for 2 × 5 min, 0.5 × SSC 37°C for 10 min, DAPI staining solution 4 (Servicebio, China) was added to the cell slices in the dark condition for 8 min. ..

Article Title: Spatiotemporal dynamics of morphology-metabolism interplay in Scutellaria baicalensis hollow root morphogenesis via spatially resolved metabolomics.
Article Snippet: Background: Understanding the morphogenetic processes and their spatial crosstalk with metabolism is essential for improving the quality of medicinal plants and constructing the regulation of the metabolite network.. However, there is a paucity of detailed research on the spatiotemporal dynamics of morphology-metabolism interplay in medicinal plants.. The roots of perennial Scutellaria baicalensis Georgi (S. baicalensis) have typical morphological characteristics (hollow root) and flavonoid metabolic heterogeneity, but the relationship between them at the spatiotemporal level has not been explored.

Labeling:

Article Title: Lactoferrin Deficiency During Lactation Causes Adult Obesity-Related Metabolic Disease Through Persistent Adipose Dysfunction Driven by Impaired Adipocyte Development.
Article Snippet: .. Cell nuclei were stained with DAPI (Servicebio, Wuhan, China, Cat. No: G1012), and cell membranes were labeled with Wheat Germ Agglutinin (WGA) (Sigma, USA, Cat. No: L4895) Crown-like structures (CLS) in adipose tissue were analyzed using ImageJ. ..

Whole Genome Amplification:

Article Title: Lactoferrin Deficiency During Lactation Causes Adult Obesity-Related Metabolic Disease Through Persistent Adipose Dysfunction Driven by Impaired Adipocyte Development.
Article Snippet: .. Cell nuclei were stained with DAPI (Servicebio, Wuhan, China, Cat. No: G1012), and cell membranes were labeled with Wheat Germ Agglutinin (WGA) (Sigma, USA, Cat. No: L4895) Crown-like structures (CLS) in adipose tissue were analyzed using ImageJ. ..

Incubation:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Fluorescence:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Microscopy:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Saline:

Article Title: Hesperidin-loaded Eudragit S100 nanoparticles alleviate ulcerative colitis by repairing intestinal barrier and modulating gut microbiota
Article Snippet: Polysorbate 80 (Tween 80) was obtained from Adamas-Beta Co., Ltd. (Shanghai, China). .. Citrate antigen retrieval buffer (pH 6.0), EDTA antigen retrieval buffers (pH 8.0 and pH 9.0), phosphate-buffered saline (PBS), tissue autofluorescence quencher, and 4′,6-diamidino-2-phenylindole (DAPI) staining reagent were provided by Servicebio Technology Co., Ltd. (Wuhan, China). .. Propidium iodide (PI) and Calcein-AM were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China).

TUNEL Assay:

Article Title: Spatiotemporal dynamics of morphology-metabolism interplay in Scutellaria baicalensis hollow root morphogenesis via spatially resolved metabolomics.
Article Snippet: Background: Understanding the morphogenetic processes and their spatial crosstalk with metabolism is essential for improving the quality of medicinal plants and constructing the regulation of the metabolite network.. However, there is a paucity of detailed research on the spatiotemporal dynamics of morphology-metabolism interplay in medicinal plants.. The roots of perennial Scutellaria baicalensis Georgi (S. baicalensis) have typical morphological characteristics (hollow root) and flavonoid metabolic heterogeneity, but the relationship between them at the spatiotemporal level has not been explored.



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Image Search Results


Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Journal: Biomaterials and Biosystems

Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

doi: 10.1016/j.bbiosy.2026.100137

Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

Techniques: Staining, Cell Culture